TABLE 1.
Details of the PCR primers used for rearrangement analysis within cag-PAI
| Primer pairs | Sequence | Amplicon size (bp) | Locus name | Coordinates in HP26695 genome |
|---|---|---|---|---|
| cagAF1 | AACAGGACAAGTAGCTAGCC | 349 | HP0547/JHP0495 | 582638-583137 |
| cagAR1 | TATTAATGCGTGTGTGGCTG | |||
| cagAF2 | GATAACAGGCAAGCTTTTGA | 701 | HP0547/JHP0495 | 580092-580440 |
| cagAR2 | TCTGCCAAACAATCTTTTGCAG | |||
| cagAP-F1 | GTGGGTAAAAATGTGAATCG | 730 | Promoter region of HP0547/JHP0495 | 579697-580440 |
| cagAR2 | TCTGCCAAACAATCTTTTGCAG | |||
| cagAP-F2 | CTACTTGTCCCAACCATTTT | 1181 | Promoter region of HP0547/JHP0495 | 579231-580440 |
| cagAR2 | TCTGCCAAACAATCTTTTGCAG | |||
| cagEF1 | GCGATTGTTATTGTGCTTGTAG | 329 | HP0544/JHP0492 | 577492-579697 |
| cagER1 | GAAGTGGTTAAAAAATCAATGCCCC | |||
| cagTF1 | CCATGTTTATACGCCTGTGT | 301 | HP0532/JHP0522 | 565171-565471 |
| cagTR1 | CCATGTTTATACGCCTGTGT | |||
| LecF1 | ACATTTTGGCTAAATAAACGCTG | 384 | JHP0521/JHP0522 | 547222-547242 |
| LecR1 | TCTCCATGTTGCCATTATGCT | |||
| LecF2 | ATAGCGTTTTGTGCATAGAA | 877 | JHP0520/JHP521 | -a |
| LecR2 | ATCTTTAGTCTCTTTAGCTT |
a
-, Ikenoue et al. (22).