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COS-1 were transfected with 200 ng/ml DNA encoding wt or C270A hASBT and pre-incubated for 10 min with buffer, 3β-Cl-CDCA (50 μM), 7α-Ms-CDCA (50 μM), or the thiol modifying-agent MTSET (1mM). Remaining hASBT activity was measure by TCA uptake. Unlike MTSET, electrophilic CDCA derivatives were able to inactivate both wt and C270A hASBT (n=3, p < 0.01).
