FIG. 5.

Epitope mapping of anti-N MAbs. Reactivity of anti-N MAbs with the recombinant N protein and peptides derived from the N protein was determined by ELISA. (A) The recombinant N protein; (B) the peptide corresponding to aa 388 to 404; (C) the peptide corresponding to aa 76 to 93; (D) the peptide corresponding to aa 84 to 101. Pooled sera from two mice immunized with inactivated SARS-CoV were used as the positive control (P), while a normal mouse serum was used as the negative control (N). The MAbs were tested at a concentration of 10 μg/ml, and the control sera were tested at a 1:100 dilution.