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. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: Dev Biol. 2016 Dec 18;422(1):4–13. doi: 10.1016/j.ydbio.2016.12.020

Figure 3. K8+ cell numbers in touch domes decrease over the first 3 weeks of postnatal life but remain constant throughout natural and induced hair cycles.

Figure 3

(A) Average K8+ cell numbers per touch dome at different stages of the natural hair cycle in back skin of C57Bl/6J female mice of varying ages (n=3–5 mice/age). Inset shows skin wholemount immunostained for Keratin 8. One-way ANOVA demonstrates no differences in average K8+ cell number per touch dome during the first hair cycle, (F=1.33, p=0.29). (B) Average K8+ cell numbers per touch dome in depilated (green) vs. undepilated (gray) back skin 0, 3, 5, 12, and 18 days post-depilation (n=3 mice/timepoint). Inset shows whole mount skin immunostained for Keratin 8. Scale bar: 50µm. Two-way ANOVA demonstrates no differences in average K8+ cell number per touch dome between induced and naturally cycling skin at any time (F=0.5848, p=0.7). Bars on graphs are SEMs.