Figure 4.

SEM imaging of myotubes on aligned micropatterned substrates. (a) SEM image of the micropatterned PDMS substrate with a continuous sinusoidal wave pattern (top view), scale bar: 50 μm. (b) SEM image of the micropatterned PDMS substrate (cross-sectional view), scale bar: 10 μm. (c) Myotubes cultured on the micropatterned substrate. Black arrow indicates longitudinal microfeature direction, scale bar: 40 μm. (d,f) Aligned cytoskeleton and nuclei of cells cultured on micropatterned substrates, scale bar: 100 μm. (e,g) Randomly oriented cytoskeleton and nuclei of cells cultured on flat PDMS substrate, scale bar: 100 μm. Reprinted with permission, Copyright © 2009, 2006 Elsevier Ltd. ^{[68, 72]}