Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2018 Feb 1.

Published in final edited form as: Biochimie. 2016 Dec 26;133:103–111. doi: 10.1016/j.biochi.2016.12.010

Proliferative and anti-proliferative effects of exogenous HCY over a wide (62.5 µM to 8 mM) concentration range on HASMC. (a) Proliferation was observed until the HCY concentration reached 1mM using MTT assay. The absorbance was monitored at 570 nm using a micro plate reader. HCY concentrations (2mM, 4mM, 8mM and 500 µM) were used. Percent viability was derived from LIVE/DEAD assay. The fluorescence was monitored at em 495/ex 530 and em 495/ex 645 using a micro plate reader. Results are expressed as the percentage of negative control mean ± SE. 1% PBS, the vehicle, served as the control. * p <0.05 versus 1% PBS.

Proliferative and anti-proliferative effects of exogenous HCY over a wide (62.5 µM to 8 mM) concentration range on HASMC. (a) Proliferation was observed until the HCY concentration reached 1mM using MTT assay. The absorbance was monitored at 570 nm using a micro plate reader. HCY concentrations (2mM, 4mM, 8mM and 500 µM) were used. Percent viability was derived from LIVE/DEAD assay. The fluorescence was monitored at em 495/ex 530 and em 495/ex 645 using a micro plate reader. Results are expressed as the percentage of negative control mean ± SE. 1% PBS, the vehicle, served as the control. * p <0.05 versus 1% PBS.