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. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: Alcohol. 2016 Nov 10;58:107–125. doi: 10.1016/j.alcohol.2016.07.011

Fig. 3. Correlations between average pregnenolone levels and ethanol intake across CIE-exposed female BXD strains.

Fig. 3

Mice from each genotype received four cycles of chronic intermittent ethanol (CIE) vapor exposure (CIE group) or air exposure (CTL group) (16 h/day × 4days, followed by 72-h withdrawal), alternated with 5-day drinking test cycles using a two-bottle (15% v/v ethanol vs. water) limited access (2 h/day) drinking model. Blood samples for pregnenolone assay were collected 72 h after a fifth CIE or air exposure cycle. Pregnenolone levels (x-axis; GeneNetwork BXD phenotype ID 17463) are expressed as pg/mL and are the average for each strain. Only data from CIE females are included in the analysis. (A) Average ethanol intake (milliliters) during Test 4 of the two-bottle choice test, Spearman r = 0.48, p = 0.045, n = 18. (B) Average ethanol intake (g/kg) during Test 4 of the two-bottle choice test, Spearman r = 0.53, p = 0.02, n = 18. (C) Absolute change in ethanol intake from baseline to Test 4, Spearman r = 0.48, p = 0.04, n = 18. (D) Percent change in ethanol intake from baseline to Test 4, Spearman r = 0.52, p = 0.03, n = 18. No significant correlations were observed between average pregnenolone levels and parameters of ethanol consumption in the other CTL or CIE cases examined.