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. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: Alcohol. 2016 Nov 10;58:107–125. doi: 10.1016/j.alcohol.2016.07.011

Table 3.

Trait correlations between serum 3α,5α-THP levels and behavioral or neurochemical phenotypes across the BXD strains.

GN ID Phenotypes Spearman
17305 3α,5α-THP in CTL males and females Reference r p n
17448 3α,5α-THP in CTL males Present results 0.90 0.0000 18
17449 3α,5α-THP in CTL females Present results 0.66 0.0030 17
17306 3α,5α-THP in CIE males and females Present results 0.69 0.0007 19
17450 3α,5α-THP in CIE males Present results 0.74 0.0001 19
17456 3α,5α-THDOC in CIE males Present results 0.77 0.0001 19
17455 3α,5α-THDOC in CIE males and females Present results 0.57 0.0100 19
17458 Pregnenolone in CTL males and females Present results 0.50 0.0320 18
13565 Ethanol (20% v/v) consumption using drinking in the dark (DID) method (4 hr access on day 4 of DID) in females,
baseline in normally housed group, control for chronic mild stress group (Phase 1) [g/kg]		 Jones B. C. et al.,
Unpublished		 0.75 0.0299 8
13576 Ethanol (20% v/v) consumption using drinking in the dark (DID) method (4 hr access on day 4 of DID) in control
females, within-group change (phase 2 minus phase 1)		 Jones B. C. et al.,
Unpublished		 −0.86 0.0041 8
12979 Alanine transaminase level in serum following alcohol (6 g/kg via gastric gavage) at 24 hours after gavage in
young adult males and females		 Rooney R. J. et al.,
Unpublished		 −0.75 0.0004 16
12985 Alanine transaminase level in serum following alcohol (6 g/kg via gastric gavage) at 24 hours after gavage in
young adult males		 Rooney R. J. et al.,
Unpublished		 −0.59 0.0143 16
12438 Anxiety, restraint stress (15 min) and ethanol (1.8 g/kg ip) (RSE group), time in open quadrants using an
elevated zero maze in 60 to 120-day-old males only during 10 min [% time]		 Cook M. et al.,
Unpublished		 0.69 0.0011 18
12436 Anxiety, restraint stress (15 min) and ethanol (1.8 g/kg ip) (RSE group), time in open quadrants using an
elevated zero maze in 60 to 120-day-old males only during first 5 min [% time]		 Cook M. et al.,
Unpublished		 0.62 0.0046 18
12366 Anxiety, ethanol treated [1.8 g/kg i.p] (NOE group), time in open quadrants using an elevated zero maze in 60 to
120-day-old females during first 5 min [% time]		 Cook M. et al.,
Unpublished		 0.49 0.0359 18
12437 Anxiety, restraint stress (15 min) and ethanol (1.8 g/kg ip) (RSE group), time in open quadrants using an
elevated zero maze in 60 to 120-day-old males only during last 5 min [% time]		 Cook M. et al.,
Unpublished		 0.54 0.0199 18
11454 Anxiety, untreated baseline, entries into closed arms of a plus maze for males [n] (Philip et al., 2010) 0.72 0.0106 11
12337 Anxiety, baseline untreated control (BASE group), time in open quadrants using an elevated zero maze in 60 to
120-day-old females only during last 5 min [percentage of time]		 Cook M. et al.,
Unpublished		 0.70 0.0008 18
12339 Anxiety, baseline untreated control (BASE group), activity in closed quadrants using an elevated zero maze in 60
to 120-day-old females only during first 5 min [n beam breaks]		 Cook M. et al.,
Unpublished		 −0.51 0.0298 18
12357 Anxiety, baseline untreated control (BASE group), time in open quadrants using an elevated zero maze in 60 to
120-day-old males and females during last 5 min [percentage of time]		 Cook M. et al.,
Unpublished		 0.68 0.0008 19
12338 Anxiety, baseline untreated control (BASE group), time in open quadrants using an elevated zero maze in 60 to
120-day-old females only during 10 min [percentage of time]		 Cook M. et al.,
Unpublished		 0.61 0.0063 18
12345 Anxiety, baseline untreated control (BASE group), latency to enter an open quadrant using an elevated zero
maze in 60 to 120-day-old males only [sec]		 Cook M. et al.,
Unpublished		 0.69 0.0009 18
12336 Anxiety, baseline untreated control (BASE group), time in open quadrants using an elevated zero maze in 60 to
120-day-old females only during first 5 min [percentage of time]		 Cook M. et al.,
Unpublished		 0.58 0.0108 18
12358 Anxiety, baseline untreated control (BASE group), time in open quadrants using an elevated zero maze in 60 to
120-day-old males and females during 10 min [percentage of time]		 Cook M. et al.,
Unpublished		 0.53 0.0193 19
12359 Anxiety, baseline untreated control (BASE group), activity in closed quadrants using an elevated zero maze in 60
to 120-day-old males and females during first 5 min [n beam breaks]		 Cook M. et al.,
Unpublished		 −0.48 0.0353 19
12417 Anxiety, saline treated [0.18 ml/kg i.p.] (NOS group), time in open quadrants using an elevated zero maze in 60
to 120-day-old males and females during last 5 min [percentage of time]		 Cook M. et al.,
Unpublished		 0.71 0.0004 19
12347 Anxiety, baseline untreated control (BASE group), time in open quadrants using an elevated zero maze in 60 to
120-day-old males only during last 5 min [percentage of time]		 Cook M. et al.,
Unpublished		 0.56 0.0142 19
12407 Anxiety, saline treated [0.18 ml/kg i.p.] (NOS group), time in open quadrants using an elevated zero maze in 60
to 120-day-old males only during last 5 min [percentage of time]		 Cook M. et al.,
Unpublished		 0.55 0.0132 19
12418 Anxiety, saline treated [0.18 ml/kg i.p.] (NOS group), time in open quadrants using an elevated zero maze in 60
to 120-day-old males and females during 10 min [percentage of time]		 Cook M. et al.,
Unpublished		 0.63 0.0028 19
11404 Open field behavior, percentage of distance in the center for males [%] (Philip et al., 2010) −0.67 0.0147 12
11419 Open field behavior, percentage of distance in the perimeter for males [%] (Philip et al., 2010) 0.67 0.0147 12
11918 Open field behavior, percentage of distance in the center for males and females [%] (Philip et al., 2010) −0.66 0.0163 12
11933 Open field behavior, percentage of distance in the perimeter for males and females [%] (Philip et al., 2010) 0.66 0.0163 12
11661 Open field behavior, percentage of distance in the center for females [%] (Philip et al., 2010) −0.59 0.0433 12
11676 Open field behavior, percentage distance in the perimeter for females [%] (Philip et al., 2010) 0.59 0.0433 12
11937 Acoustic startle response, maximum response at 70 db for males and females (65 dB background noise,
Hamilton-Kinder SM100 startle chamber, 10 msec interval, 120 dB startle stimulus, prepulse PPI control data by
M Cook, UMemphis) [force]		 (Philip et al., 2010) −0.72 0.0064 12
11425 Acoustic startle response, maximum response at 85 db for males (65 dB background noise, Hamilton-Kinder
SM100 startle chamber, 10 msec interval, 120 dB startle stimulus, prepulse PPI control data by M Cook,
UMemphis) [force]		 (Philip et al., 2010) −0.83 0.0003 12
11939 Acoustic startle response, maximum response at 85 db for males and females (65 dB background noise,
Hamilton-Kinder SM100 startle chamber, 10 msec interval, 120 dB startle stimulus, prepulse PPI control data by
M Cook, UMemphis) [force]		 (Philip et al., 2010) −0.83 0.0004 12
11423 Acoustic startle response, maximum response at 70 db for males (65 dB background noise, Hamilton-Kinder
SM100 startle chamber, 10 msec interval, 120 dB startle stimulus, prepulse PPI control data by M Cook,
UMemphis) [force]		 (Philip et al., 2010) −0.73 0.0056 12
11424 Acoustic startle response, maximum response at 80 db for males (65 dB background noise, Hamilton-Kinder
SM100 startle chamber, 10 msec interval, 120 dB startle stimulus, prepulse PPI control data by M Cook,
UMemphis) [force]		 (Philip et al., 2010) −0.74 0.0042 12
11938 Acoustic startle response, maximum response at 80 db for males and females (65 dB background noise,
Hamilton-Kinder SM100 startle chamber, 10 msec interval, 120 dB startle stimulus, prepulse PPI
control data by M Cook, UMemphis) [force]		 (Philip et al., 2010) −0.80 0.0011 12
11011 Fear conditioning, freezing response to context after 48 hours [%] (Brigman et al., 2009) −0.89 0.0041 7
11823 Pain response, mechanical nociception, tail clip latency for males and females [sec] (Philip et al., 2010) 0.62 0.0288 12
11816 Pain sensitivity, vocalization threshold to mild foot shock for males and females [mA] (Philip et al., 2010) 0.63 0.0263 12
11269 Adrenal zona glomerulosa width for females [µm] (Di Curzio & Goldowitz, 2011) 0.66 0.0083 14
17306 3α,5α-THP in CIE males and females
17450 3α,5α-THP in CIE males Present results 0.84 0.0000 19
17448 3α,5α-THP in CTL males Present results 0.75 0.0002 18
17305 3α,5α-THP in CTL males and females Present results 0.69 0.0007 19
17451 3α,5α-THP in CIE females Present results 0.63 0.0080 16
13565 Ethanol (20% v/v) consumption using drinking in the dark (DID) method (4 hr access on day 4 of DID) in females,
baseline in normally housed group, control for chronic mild stress group (Phase 1) [g/kg]		 Jones B. C. et al.,
Unpublished		 0.71 0.0452 8
12369 Anxiety, ethanol treated [1.8 g/kg i.p] (NOE group), activity in closed quadrants using an elevated zero maze in
60 to 120-day-old females only during first 5 min [n beam breaks]		 Cook M. et al.,
Unpublished		 −0.70 0.0007 18
12371 Anxiety, ethanol treated [1.8 g/kg i.p] (NOE group), activity in closed quadrants using an elevated zero maze in
60 to 120-day-old females only during 10 min [n beam breaks]		 Cook M. et al.,
Unpublished		 −0.74 0.0002 18
12374 Anxiety, ethanol treated (1.8 g/kg ip) (NOE group), activity in closed quadrants using an elevated zero maze in
60 to 120-day-old females only during 10 min [beam breaks/sec]		 Cook M. et al.,
Unpublished		 0.58 0.0101 18
12370 Anxiety, ethanol treated [1.8 g/kg i.p] (NOE group), activity in closed quadrants using an elevated zero maze in
60 to 120-day-old females only during last 5 min [n beam breaks]		 Cook M. et al.,
Unpublished		 −0.72 0.0004 18
12373 Anxiety, ethanol treated [1.8 g/kg i.p] (NOE group), activity in closed quadrants using an elevated zero maze in
60 to 120-day-old females only during last 5 min [beam breaks/sec]		 Cook M. et al.,
Unpublished		 0.58 0.0104 18
12389 Anxiety, ethanol treated [1.8 g/kg i.p] (NOE group), activity in closed quadrants using an elevated zero maze in
60 to 120-day-old males and females during first 5 min [n beam breaks]		 Cook M. et al.,
Unpublished		 −0.47 0.0421 19
12396 Anxiety, saline treated [0.18 ml/kg i.p.] (NOS group), time in open quadrants using an elevated zero maze in 60
to 120-day-old females only during first 5 min [percentage of time]		 Cook M. et al.,
Unpublished		 0.66 0.0019 18
12337 Anxiety, baseline untreated control (BASE group), time in open quadrants using an elevated zero maze in 60 to
120-day-old females only during last 5 min [percentage of time]		 Cook M. et al.,
Unpublished		 0.56 0.0151 18
12418 Anxiety, saline treated [0.18 ml/kg i.p.] (NOS group), time in open quadrants using an elevated zero maze in 60
to 120-day-old males and females during 10 min [percentage of time]		 Cook M. et al.,
Unpublished		 0.56 0.0114 19
12398 Anxiety, saline treated [0.18 ml/kg i.p.] (NOS group), time in open quadrants using an elevated zero maze in 60
to 120-day-old females only during 10 min [percentage of time]		 Cook M. et al.,
Unpublished		 0.60 0.0070 18
12359 Anxiety, baseline untreated control (BASE group), activity in closed quadrants using an elevated zero maze in 60
to 120-day-old males and females during first 5 min [n beam breaks]		 Cook M. et al.,
Unpublished		 −0.57 0.0096 19
12417 Anxiety, saline treated [0.18 ml/kg i.p.] (NOS group), time in open quadrants using an elevated zero maze in 60
to 120-day-old males and females during last 5 min [percentage of time]		 Cook M. et al.,
Unpublished		 0.63 0.0033 19
12345 Anxiety, baseline untreated control (BASE group), latency to enter an open quadrant using an elevated zero
maze in 60 to 120-day-old males only [sec]		 Cook M. et al.,
Unpublished		 0.59 0.0094 18
12397 Anxiety, saline treated (0.18 ml/kg ip, NOS group), time in open quadrants only during last 5 min of test using an
elevated zero maze for 60- to 120-day-old females [%]		 Cook M. et al.,
Unpublished		 0.53 0.0231 18
12339 Anxiety, baseline untreated control (BASE group), activity in closed quadrants using an elevated zero maze in 60
to 120-day-old females only during first 5 min [n beam breaks]		 Cook M. et al.,
Unpublished		 −0.50 0.0332 18
12361 Anxiety, baseline untreated control (BASE group), activity in closed quadrants using an elevated zero maze in 60
to 120-day-old males and females during 10 min [n beam breaks]		 Cook M. et al.,
Unpublished		 −0.52 0.0219 19
11455 Anxiety, untreated baseline, entries into open arms of a plus maze for males [n] (Philip et al., 2010) 0.63 0.0371 11
11969 Anxiety, untreated baseline, entries into open arms of a plus maze for males and females [n] (Philip et al., 2010) 0.72 0.0106 11
11936 Acoustic startle response, maximum response at 120 db for males and females (65 dB background noise,
Hamilton-Kinder SM100 startle chamber, 10 msec interval, 120 dB startle stimulus, prepulse PPI control data by
M Cook, UMemphis) [force]		 (Philip et al., 2010) −0.68 0.0132 12
11937 Acoustic startle response, maximum response at 70 db for males and females (65 dB background noise,
Hamilton-Kinder SM100 startle chamber, 10 msec interval, 120 dB startle stimulus, prepulse PPI control data by
M Cook, UMemphis) [force]		 (Philip et al., 2010) −0.67 0.0147 12
11679 Acoustic startle response, maximum response at 120 db for females (65 dB background noise, Hamilton-Kinder
SM100 startle chamber, 10 msec interval, 120 dB startle stimulus, prepulse PPI control data by M Cook,
UMemphis) [force]		 (Philip et al., 2010) −0.59 0.0433 12
11422 Acoustic startle response, maximum response at 120 db for males (65 dB background noise, Hamilton-Kinder
SM100 startle chamber, 10 msec interval, 120 dB startle stimulus, prepulse PPI control data by M Cook,
UMemphis) [force]		 (Philip et al., 2010) −0.64 0.0219 12
11423 Acoustic startle response, maximum response at 70 db for males (65 dB background noise, Hamilton-Kinder
SM100 startle chamber, 10 msec interval, 120 dB startle stimulus, prepulse PPI control data by M Cook,
UMemphis) [force]		 (Philip et al., 2010) −0.61 0.0341 12
11938 Acoustic startle response, maximum response at 80 db for males and females (65 dB background noise,
Hamilton-Kinder SM100 startle chamber, 10 msec interval, 120 dB startle stimulus, prepulse PPI control data by
M Cook, UMemphis) [force]		 (Philip et al., 2010) −0.64 0.0241 12
11424 Acoustic startle response, maximum response at 80 db for males (65 dB background noise, Hamilton-Kinder
SM100 startle chamber, 10 msec interval, 120 dB startle stimulus, prepulse PPI control data by M Cook,
UMemphis) [force]		 (Philip et al., 2010) −0.62 0.0313 12
11295 Adrenal zona reticularis width for males [µm] (Di Curzio & Goldowitz, 2011) 0.59 0.0257 14

Variation in 3α,5α-THP levels across the BXD population was linked to behavioral or neurochemical phenotypes previously characterized across these strains by other laboratories and whose data are available in GeneNetwork. GN ID refers to the GeneNetwork BXD phenotype ID for each trait.