Figure 4.

Reproductivity and immunogenicity of EmagER. Three groups of rabbits were inoculated with either 200 oocysts of Eimeria magna wild-type (EmagWT), transgenic E. magna-expressing enhanced yellow fluorescent protein and red fluorescent protein (EmagER), or 200 µl PBS, respectively. (A) Daily oocyst output of individual rabbit during patent period were measured using a McMaster counting chamber. Fourteen days later, a challenge of 1 × 10⁴ oocysts of EmagWT was performed to all rabbits. Body weight gain (B) and total oocyst output (C) were measured in 14 days post challenge. UUC, unimmunized and unchallenged control; UCC, unimmunized and challenged control. All data were presented as mean ± SEM values. Statistical analysis was performed by one-way ANOVA, LSD, *p < 0.05, and **p < 0.01.