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. 2017 Jan 23;4:99. doi: 10.3389/fbioe.2016.00099

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Copyright © 2017 Chicaybam, Barcelos, Peixoto, Carneiro, Limia, Redondo, Lira, Paraguassú-Braga, Vasconcelos, Barros and Bonamino.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Sleeping beauty-based GFP gene transfer to adipose tissue derived human mesenchymal stem cells (MSCs). (A) MSCs were electroporated with each one of the seven buffers and the recommended program. Viability (blue bar), GFP expression (green bar), and electroporation score (red bar) were assessed 1 day after nucleofection (d + 1). (B) Long-term GFP expression was evaluated until d + 30 post nucleofection with (white bar) or without (black bar) the addition of SB100× transposase (1 μg per cuvette). Data are shown as mean ± SD from three experiments performed in duplicate and were further analyzed using one-way ANOVA with Tukey’s multiple comparisons test. Significant differences (p < 0.05) are depicted in the table next to each graph, with each color denoting one parameter.