Figure 3.

Characterization of ohp1 and ohp2 mutants. (A) Representative images of 3-week-old homozygous (hom) or heterozygous (het) ohp1-1 and ohp2-1 mutants in comparison to Col-0 WT plants of the same age. The plants were grown in axenic culture with continuous light. (B) Northern blot analysis of OHP1 and OHP2 transcript levels in the respective homozygous or heterozygous mutants in comparison to WT Col-0 plants. Fifteen microgram of total RNA were loaded per lane and EtBR-stained 25S rRNA is shown as loading control. (C) Western blot analysis of OHP1 and OHP2 protein levels in corresponding samples. Ten microgram of LDS-solubilized insoluble proteins were loaded per lane and Coomassie-stained LHC proteins are shown as loading control. (D) Pigments from leaves of Col-0 WT plants or heterozygous and homozygous ohp1-1 mutants were extracted with 80% acetone and quantified photometrically. Values represent the mean±SD (N = 4), Asterisks indicate significant differences from the respective WT value (p ≤ 0.01 by Student's t-test). (E) Pigments of homozygous ohp1-1 mutants and WT seedlings separated by thin layer chromatography.