Figure 8.
Complementation of ohp mutants. (A,B) Photos and Fv/Fm images of 2-week-old WT plants, homozygous ohp1-1 or ohp2-1 mutants and complemented mutants (T2 generation). Color scale for Fv/Fm is shown between (A,B). In the T1 generation after transformation of heterozygous ohp mutants with OHP overexpression constructs, plants homozygous or heterozygous for the ohp1-1 or ohp2-1 T-DNA insertions were identified by PCR. T2 plants were selected for BASTA resistance conferred by the overexpression constructs, sulfadiazine resistance conferred by the KO-constructs and transferred to plates without herbicide 5 days after germination. (C) Transcript analysis of plants from the cross-complementation attempt. Transcripts of the rescue constructs (35S-OHP1 and 35S-OHP2) can be distinguished from endogenous transcripts by their bigger size due to artificial UTRs. Fifteen microgram of total RNA were loaded per lane and EtBR-stained 25S rRNA is shown as loading control.