Table 2.

A selection of 9 genes of particular interest because of their novelty and possible roles as markers or as neuromodulators (for an extended list of genes see Table 1) has been confirmed to be expressed in the transcriptome of the parvafox nucleus by qRT-PCR.

	Gene-array	qRT PCR*	B*	ABA	IC	Literature
Cbln1	x	x	x	x
Drd2	x	x	x	x
Ephb1	x	x		x
Foxb1	x	x		neg	x**	x
Glra1	x	x	x	x
Glra2	x	x	x	x	x
Glra3	x	x	x	x
Npb	x	x		neg		x
Npsr1	x	x	No data	x

We also examined the expression of these 9 genes in the BrainStars (B^*) database and the ABA-ISH database, additionally we performed immunohistochemistry (IC) to validate the expression of Glra2 and Foxb1. Glra2 satisfies all five criteria for expression in the parvafox nucleus and represents the most robust result of this study. Cerebellin (Cbln1) and the dopamine 2 receptor (Drd2) meet four of the five criteria for occurrence in the parvafox nucleus.

Foxb1 and Npb are positive in our own gene-array and have been revealed by others (Dun et al., 2005; Civelli et al., 2006) to occur in the lateral hypothalamus, respectively in the parvafox nucleus (Bilella et al., 2014) but could not be confirmed neither in the ABA-ISH nor in the B^* databases.

^*p values calculated in Student's t-test (ΔCt of both nuclei vs. both cortex and hippocampus).

^**Positive in Foxb1-Cre-Egfp knock-in mice (Bilella et al., 2014).