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. 2017 Jan 23;3:16100. doi: 10.1038/cddiscovery.2016.100

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Copyright © 2017 The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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SNX2 is a caspase-6 substrate. (a) HeLa cells were transfected with control (siCTL) or caspase-6 small interfering RNAs (siRNAs) (siC6) followed by a 16 h treatment with TRAIL (100 ng/ml), STS (0.5 μM) or UV (254 nm wavelength; 100 J/m²). (b) Eighty micrograms of HeLa cell extracts were left untreated or incubated with 250 nM active site-titrated recombinant caspase-6 for an hour at 37 °C. (c) HEK293T cells were transfected with either control (−), Flag-tagged WT or catalytic mutant (C285A) caspase-6 plasmids for 48 h. Proteins were analyzed by immunoblotting using the indicated antibodies. Closed and open arrowheads indicate full-length and cleaved fragments, respectively. Actin and HSP90 were used as loading controls. The asterisk indicates a nonspecific protein recognized by the SNX2 antibody.