Figure 8.

Overexpression of AhRRS5 enhanced resistance to Ralstonia solanacearum in transgenic tobacco. (a) Schematic of the pBI121‐AhRRS5 construct. LB and RB, left and right borders of the T‐DNA; 2 × 35SPro, two cauliflower mosaic virus 35S promoters; Nos‐T, NOS terminator; Kan^r, kanamycin resistance. (b) The third leaves of 8‐week‐old wild‐type tobacco and AhRRS5‐OE‐3 transgenic plants were inoculated with 10 μL of suspension of 10⁸ cfu per millilitre of high‐virulence R. solanacearum strain. The photograph was obtained 20 days postinoculation (dpi). (c) RT‐PCR analysis of AhRRS5 expression in transgenic and wild‐type tobacco plants; expression level of Ntactin was visualized as endogenous control. (d) Disease symptoms of detached leaves of wild‐type and AhRRS5‐OE‐3 transgenic plants after inoculation with R. solanacearum. Transgenic leaves showed immune resistance or high‐resistance phenotype. Photos were obtained at 7 and 20 dpi. (e) Different phenotypes of the stem were observed between wild‐type and transgenic AhRRS5‐OE‐3 plants after inoculation with R. solanacearum. Transgenic plant stem showed no or much week infections. Photos were taken at 7 and 20 dpi.