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. 2017 Jan 9;2017:9251303. doi: 10.1155/2017/9251303

Figure 3.

Figure 3

Arsenite effect on mitochondrial respiratory complex expression and activity in primary hepatocytes. ((a) and (c)) Immunoblot analysis of mitochondrial respiratory complex expression in (a) mouse and (c) human primary hepatocytes. Histograms to the right of (a) and (c) show ImageJ analysis of the respective immunoblots. ((b) and (d)) Complex I activity in (b) mouse and (d) human primary hepatocytes. Tables to the right of (b) and (d) provide statistical analysis of Complex I activity data, respectively. Results representative of four independent experiments. Complex I activity data presented as mean ± SD. ImageJ analysis data are normalized to actin and presented as mean ± SD. ∗Significantly different from vehicle and 0–2.5 μM arsenite treated hepatocytes; P < 0.01. #Significantly different from 5 μM arsenite treated hepatocytes; P < 0.01. C-I, Complex I; C-IV, Complex IV; C-III, Complex III; C-V, Complex V.