Skip to main content
. 2017 Jan 9;2017:7316354. doi: 10.1155/2017/7316354

Figure 1.

Figure 1

Kindlin-2 expression pattern, overexpression/knockdown, and targets. (a) mRNA expression levels of Kindlin-2 in F134 iPSCs and different passages of iPSC-MSCs indicated significant differences with BM-MSCs; total proteins were extracted from all cell types. (b) Western blotting was performed using an anti-Kindlin-2 monoclonal antibody. (c) Real-time qPCR was performed to quantify mRNA levels of Kindlin-2 in iPSC-MSCs (passages 1–3) with Kindlin-2 overexpression or knockdown. (d, e) Western blot analysis (d) and densitometry (e) performed with ImageJ software were performed and indicate efficient overexpression and knockdown. Data represents the mean expression values normalized to the housekeeping gene GAPDH. ∗Significance difference p ≤ 0.05. ∗∗Significance difference p ≤ 0.01.