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. 2017 Jan 23;8:4. doi: 10.3389/fmicb.2017.00004

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Copyright © 2017 Dotto, Lombarte Serrat, Cattelan, Barbagelata, Yantorno, Sordelli, Ehling-Schulz, Grunert and Buzzola.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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SAL chelating capacity of iron. (A) Standard curve of ferrous ion concentration in ferrozine complexes determined in TSBg medium using 1.25 mM ferrozine. (B) Ferrozine-Fe²⁺ complex quantification in TSBg treated with different concentrations of SAL mixed with 50 μM FeSO₄. The amount of ferrozine-Fe²⁺ complex was determined at an absorbance of 540 nm (Abs₅₄₀). Basal iron concentration in TSBg was 39.3 μM.