Figure 4.

The effect of SAL on biofilm formed by MRSA and MSSA strains. Biofilms were formed in TSBg during 24 h in the presence or absence of 2 mM of SAL or SAL plus 50 μM of FeSO₄. Each bar represents the arithmetic mean ± SEM of 6 wells from 4 independent experiments. The biofilms were quantified by crystal violet staining (OD_B) and expressed relative to the final culture density (OD_G). Statistically significant differences were represented by asterisks: (^*) SAL-treated group vs. untreated group; (^**) SAL-treated group vs. SAL plus iron-treated group. p < 0.05 were considered significant (Mann-Whitney test).