Table 1.
Strain | Spa type | MLST | SCCmec | Course of infection | |
---|---|---|---|---|---|
CC | ST | ||||
CBS | t149 | 5 | 5 | I | – |
BRZ | t138 | 8 | 239 | II | – |
AR48 | t149 | 5 | 5 | I | Chronic |
AR66 | t002 | 5 | 100 | IV | Acute |
AR94 | t008 | 8 | 8 | IV | Chronic |
AR56 | t149 | 5 | 5 | – | Chronic |
AR70 | t002 | 5 | 5 | – | Acute |
AR83 | t002 | 5 | 5 | – | Chronic |
Newman | t008 | 8 | 8 | – | – |
S. aureus strains related to USA300 (CC8) or USA100 (CC5) clones were chosen. CBS and BRZ strains are representatives of the Cordobés and the Brazilian HA-MRSA clones, respectively. S. aureus AR isolates come from different hospitals of Buenos Aires City, Argentina. Multilocus sequence types (MLST), clonal complexes (CC) and sequence types (ST) were determined previously. The defining feature of MRSA is the presence of the staphylococcal cassette chromosome mec (SCCmec). Different SCCmec types were established by specific PCR assays. The spa types from sequences of the protein A (spa) repeat region were assessed from the Ridom spa server (http://spa.ridom.de) (Lattar et al., 2012).