Figure 2.

MAPK/NF-κB signaling pathways involved on cytokine production in dendritic cells (DCs) after stimulation with Brucella abortus or bacterial RNA. DCs from C57BL/6 mice were pretreated for 45 min with increasing concentrations (in micromolars) of U0126, SB203580, SP600125, and BAY11-7082 (ERK1/2, p38, JNK, and NF-κB inhibitors, respectively) or vehicle, as indicated, and then stimulated with B. abortus (MOI 100:1) (A,C,E) or bacterial RNA (2 µg/mL) (B,D). Supernatants were harvested after 24 h after stimulation and cytokine secretion was determined by ELISA. Abbreviations: NI, non-infected; C, Control, DOTAP only;, RNA, DOTAP + RNA. Significant differences in relation to vehicle treatment are denoted (for *p < 0.05, **p < 0.01, and ***p < 0.001, two-way ANOVA test).