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. 2017 Jan 18;8:14090. doi: 10.1038/ncomms14090

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Copyright © 2017, The Author(s)

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(a) The conserved four WD40 domains in huWdr8. Mutations for WD mutant variants are denoted in red. (b) External phenotypes of WD mutant-injected Wdr8^−/− zygotes and embryos. Note that both variants mostly localised in the cytoplasm (2.6 hpf), unable to rescue Wdr8^−/− embryos (1.5 dpf). (c) Time-lapse images of EYFP-196/197AA localisation in Wdr8^−/− blastomeres. EYFP-196/197AA weakly localised to the centrosome with multiple foci, which disappeared during cleavages. Time, min. (d,e), In comparison with EYFP–huWdr8 wild-type, expression of either mutant variant was unable to rescue abnormal PCM assembly (γ-tubulin/PCM1), multipolar mitotic spindles, and chromosome alignment defects. Scale bars, 200 μm (b), 100 μm (c) and 10 μm (d,e). Stages are denoted as hours/days post fertilisation (hpf, dpf) and the corresponding stage of WT are denoted in parentheses.