Figure 3. The roles of PI3K and AMPK in PTS-induced hormetic effect in PC12 cells.

PC12 cells were exposed to 0.12 mg/ml PTS for 24 h with or without pretreatment of LY294002 (10 μM, 1 h) (A) or CC (5 μM, 1 h) (B). The protein levels of P-mTOR, mTOR, SIRT1, P-FOXO3 and FOXO3 were detected by Western blot. (C–F) were densitometric analysis of (A or B) from three experiments. **P < 0.01, compared to PTS-treated alone groups.