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. 2017 Jan 23;7:41063. doi: 10.1038/srep41063

Table 2. Bacterial strains and plasmids.

Bacterial strains/plasmids Description Source/Reference
Strain
 H. pylori G27 wild type Clinical isolate, wild type 42
 E. coli DH-5α supE44 ΔlacU169 (ϕ80 lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 43
Plasmid
 pGEM-T-Easy Cloning vector; Ampr. Promega
 pGEM0703 pGEM-T-Easy derivative, containing a 437 bp DNA fragment corresponding to the region from 722.774 to 723.211 of H. pylori G27 genome amplified by PCR with oligonucleotides 0703FPF/0703FPR. This region corresponds to a portion of the coding sequence of HPG27_RS03405 (HP0703 according to 26695 annotation). This work
 pGEMp1203 pGEM-T-Easy derivative, containing a 302 bp DNA fragment corresponding to the region from 1.268.274 to 1.268.576 of H. pylori G27 genome amplified by PCR with oligonucleotides 1203FPF/1203FPR. This region encompasses the putative promoter region of HPG27_RS06000 (HP1203 according to 26695 annotation). This work
 pGEMp1227 pGEM-T-Easy derivative, containing a 308 bp DNA fragment corresponding to the region from 1.290.538 to 1.290.846 of H. pylori G27 genome amplified by PCR with oligonucleotides 1227FPF/1227FPR. This region encompasses the putative promoter region of HPG27_RS06145 (HP1227 according to 26695 annotation). This work
 pGEMsRNA17_18 pGEM-T-Easy derivative, containing a 426 bp DNA fragment corresponding to the region from 16.304 to 16.730 of H. pylori G27 genome amplified by PCR with oligonucleotides sRNA17_18FPF/sRNA17_18FPR. This region encompasses the intergenic region between putative SRP_RNA and HPG27_RS00110. This work
 pGEMA1.4 pGEM-T-Easy derivative, containing a 199 bp DNA fragment corresponding to the region from 1.576.846 to 1.577.045 of H. pylori G27 genome amplified by PCR with oligonucleotides A1.4FPF/A1.4FPR. This region encompasses the putative promoter region of isoA toxin/antitoxin system. This work
 pGEMp0088 pGEM-T-Easy derivative, containing a 275 bp DNA fragment corresponding to the region from 94.285 to 94.560 of H. pylori G27 genome amplified by PCR with oligonucleotides 0088FPF/0088FPR. This region encompasses the putative promoter region of HPG27_RS00460 (HP0088 according to 26695 annotation). This work
 pGEMp1296 pGEM-T-Easy derivative, containing a 477 bp DNA fragment corresponding to the region from 1.362.205 to 1.362.682 of H. pylori G27 genome amplified by PCR with oligonucleotides 1296FPF/1296FPR. This region encompasses the putative promoter region of HPG27_RS06525 (HP1297 according to 26695 annotation). This work
 pGEMp1260 pGEM-T-Easy derivative, containing a 242 bp DNA fragment corresponding to the region from 1.321.773 to 1.322.015 of H. pylori G27 genome amplified by PCR with oligonucleotides 1260FPF/1260FPR. This region encompasses the putative promoter region of HPG27_RS06315 (HP1260 according to 26695 annotation). This work
 pGEM0703HY pGEM-T-Easy derivative, containing a 117 bp DNA fragment corresponding to the region from 722.983 to 723.100 of H. pylori G27 genome amplified by PCR with oligonucleotides 0703HYF/0703HYR. This region corresponds to a portion of the coding sequence of HPG27_RS03405 (HP0703 according to 26695 annotation). This work
 pGEMp1203HY pGEM-T-Easy derivative, containing a 148 bp DNA fragment corresponding to the region from 1.268.391 to 1.268.539 of H. pylori G27 genome amplified by PCR with oligonucleotides 1203HYF/1203HYR. This region encompasses the putative promoter region of HPG27_RS06000 (HP1203 according to 26695 annotation) This work
 pGEMp1227HY pGEM-T-Easy derivative, containing a 146 bp DNA fragment corresponding to the region from 1.290.664 to 1.290.810 of H. pylori G27 genome amplified by PCR with oligonucleotides 1227HYF/1227HYR. This region encompasses the putative promoter region of HPG27_RS06145 (HP1227 according to 26695 annotation) This work
 pGEMp0088HY pGEM-T-Easy derivative, containing a 136 bp DNA fragment corresponding to the region from 94.431 to 94.567 of H. pylori G27 genome amplified by PCR with oligonucleotides 0088HYF/0088HYR. This region encompasses the putative promoter region of HPG27_RS00460 (HP0088 according to 26695 annotation). This work
 pGEMpsRNA17_18HY pGEM-T-Easy derivative, containing a 129 bp DNA fragment corresponding to the region from 16.516 to 16.645 of H. pylori G27 genome amplified by PCR with oligonucleotides sRNA17_18HYF/sRNA17_18HYR. This region encompasses the intergenic region between putative SRP_RNA and HPG27_RS00110. This work
 pGEMpA1.4HY pGEM-T-Easy derivative, containing a 189 bp DNA fragment corresponding to the region from 1.576.888 to 1.577.077 of H. pylori G27 genome amplified by PCR with oligonucleotides A1.4HYF/A1.4HYR. This region encompasses the putative promoter region of isoA toxin/antitoxin system. This work