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. 2017 Jan 18;8:14100. doi: 10.1038/ncomms14100

Figure 2. Development of heat-shock-labelled cells is normal.

Figure 2

(a) Time-lapse imaging of infrared-laser labelled and promoter-labelled cells for axon-outgrowth comparisons. Asterisk, nonspecific expression from recombinase activity. Scale bar, 5 μm. (b) Timing of axon initiation and growth is not affected by heat-shock induction. Axon length plotted against developmental stage in infrared-laser-labelled ASI, AWA, ASG, AIB neurons and promoter-labelled ASI, AWA and ASG neurons. n=4 embryos per reporter. (c) Post-embryonic fate marker expression and axon trajectory in the AWA neuron are not affected by heat shock (HS). Induction performed at AB64 in ABplaapa. Animals scored at L3/L4 stages. Scale bar, 10 μm. (d) Cell-division timing is modestly delayed in heat-shocked cells. Timing of final division of heat-shocked cells compared with contralateral homologues using the symmetric UNC-130-GFP marker. Measurements in the ‘no heat shock' condition are absolute differences between left and right sides. n>8 embryos for each.