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. 2017 Jan 23;3:16105. doi: 10.1038/cddiscovery.2016.105

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Copyright © 2017 The Author(s)

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Hypoxia increased the expression of putative CSC markers. (a) In sorted A549 cells, the proportion of CD133-positive cells was only 5.59%±2.272 in the control cells, and this proportion increased in a time-dependent manner under hypoxic conditions. After 15 days of hypoxia, the proportion of CD133-positive cells reached 49.2%±3.125. The CD133 expression in sorted HepG2 cells also increased from 2.04%±1.253 to 40.6%±2.871 after 15 days of hypoxia. For GL261 cells, the proportion of CD133-positive cells was 7.03%±3.425 in the normoxia control cells, and this rate increased remarkably from 9 days to 15 days of hypoxia exposure from 20.3%±2.547 to 97.6%±3.791. In addition, the results also showed that the expression of two other stem cell markers, CD15 and NESTIN, was upregulated, increasing from 0.56%±0.251 to 60.2%±3.472 and 3.96%±5.231 to 60.3%±5.284, respectively, after 15 days of hypoxia. (b) The proportion of stem cell markers in sorted cells increased significantly under hypoxic conditions (*P<0.05).