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. 2017 Jan 19;8:14234. doi: 10.1038/ncomms14234

Figure 1. 46B8 blocks low pH-induced membrane fusion and conformational changes of HA.

Figure 1

(a) Hela cells expressing the B/Wisconsin/1/2010 HAs were treated with trypsin to activate HA0 and then incubated with either 46B8 or a control IgG before pH drop to 4.8 to induce cell–cell fusion. After overnight culture, representative images were obtained under a phase contrast microscope. Scale bar is 200 μm. (b) 293 T cells expressing the B/Victoria/504/2000 HA were treated with trypsin to activate HA0 and incubated with either 46B8 or a control IgG before pH drop to 4.8 to induce HA conformational changes. Cells were then treated with DTT (+DTT) or PBS (−DTT) and tested for 34B5 binding by flow cytometry. The mean fluorescence intensities were normalized to the PBS-treated cells in each group and the percent of binding is shown. Possible HA conformations at each stage are depicted above the binding data. HA1 is in blue and HA2 is in red.