Figure 4. Distinct sequence elements in the transit peptide of PPDK correlate with either general or P-specific chloroplasts import.

(A) Sequences of WT_PPDK and corresponding alanine substitution mutants. CS – cleavage site; PPDK – Pyruvate, Pi dikinase. (B) Quantification of GFP signals in transfected protoplasts (WT_PPDK; I – IX mutant GFP-fusion constructs) by fluorescence microscopy. Expression patterns were categorized and analyzed as described in Fig. 3 (n = 3). p-values are indicated in the figure.