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. 2017 Jan 23;7:41187. doi: 10.1038/srep41187

Figure 6. Four amino acids in the transit peptides of TPI and PPDK are necessary for P-specificity.

Figure 6

(A–E) Sequences, quantification and representative images for TPI. (F–H) Sequences, quantification and representative images for PPDK. (A,F) Sequences of the wild type TPI and PPDK transit peptide sequences and the corresponding alanine substitution mutants. (B,D,G) Quantification of GFP signals in transfected protoplasts by fluorescence microscopy. Protoplasts were analyzed (n = 3) and statistics were performed as described in Fig. 3. Asterisks indicate significant differences from the corresponding control experiment (WT_TPI or WT_PPDK) by Student’s t-test (*p < 0.05; **p < 0.01; ***p < 0.001). (C,E,H) Representative confocal images of observed phenotypes. For representative images of the phenotypes of WT_TPI and WT_PPDK see Fig. 2. For representative images of the phenotypes of motifs VII and VIII of TPI see Fig. 3).