TABLE 1.
Effects of different treatments on the adherence of C. albicans and S. cerevisiae expressing Als5p to PEG beads with KLRIPSV synthesized on their surfaces
| Chemical agent (concn) | Scorea with:
|
||
|---|---|---|---|
| C. albicans + peptide-coated PEG beads + reagent | C. albicans + peptide-coated PEG beads + yeast cells + addition of reagent after 30 min (desorption) | S. cerevisiae expressing Als5p + peptide-coated PEG beads + reagent | |
| None (control) | +++ | +++ | +++ |
| Fn/23 (100 μg/ml) | 0 | + to ++ | 0 |
| Formamide (50% [vol/vol]) | 0 | 0 to + | 0 |
a
0, no adherence; +, adherence of some yeast cells (20 to 25/bead); ++, bead almost covered by yeast cells (80 to 150/bead); +++, adherence with cell-cell aggregation.