Figure 1.

Technical reproducibility of replicates frozen immediately (A) and frozen after incubation at room temperature for 4 days (B) for the evaluation of the relative abundance of 3 phyla, 2 alpha diversity metrics, and the first principal coordinate of 4 beta diversity metrics using intraclass correlation coefficients, Mayo Clinic, Rochester, Minnesota, 2014. White columns indicate replicates stored in no solution, striped columns indicate those stored in fecal immunochemical test tubes, gray columns indicate those stored on fecal occult blood test cards, dotted columns indicate those stored in RNAlater Stabilization Solution (Ambion, Austin, Texas), and black columns indicate those stored in 95% ethanol. BC, Bray-Curtis distance; OTU, operational taxonomic unit; PC1, principal coordinate analysis component 1; SDI, Shannon diversity index.