TABLE 1.

Pyrimidine salvage pathway genes analyzed and primers used in this study^a

Gene	Gene product	Primers for PCR	Amplicon size (bp)	Primers for sequencing	Size of ORF (bp)	Introns	Intron size (bp)	No. of amino acid residues
FCY21	Cytosine permease	For 5′-TATAATTCTCCCATCACCATC-3′	1,847	For 5′-GGTGCCACTTTAGCCATTTG-3′	1,545	0		514
		Rev 5′-ACCGCTGCTTCTCTCTCCAC-3′		Rev 5′-GTCTTAATCCTAAAGCTGATCC-3′ For 5′-CTAGTAAATCATTTGAAGGTGGTG-3′
				Rev 5′-CAAGCAGCACCCAATATTAAAG-3′
FCY22	Cytosine permease	For 5′-CCCTTCACTCCAACTCTTTCC-3′	1,686	For 5′-CCCTTCACTCCAACTCTTTCC-3′	1,581	0		526
		Rev 3′-CAACATTTAAATAACACGGCAGAATG-3′		Rev 3′-CAACATTTAAATAACACGGCAGAATG-3′
				For 5′-TCTGGTCCAACTGAAGCTGG-3′
				Rev 5′-GGTAATGATAACCCGGTGGTC-3′
FCA1	Cytosine deaminase	For 5′-GTCACTTCAAAGTACCAATCTTTA-3′	633	For 5′-GTCACTTCAAAGTACCAATCTTTA-3′	523	1	70	150
		Rev 3′-GTTTAAGTTTTATATTACACTAATCTG-3′		Rev 3′-GTTTAAGTTTTATATTACACTAATCTG-3′
FUR1	UPRT	For 5′-GTCCCAATTTCTTCATCATG-3′	911	For 5′-GTCCCAATTTCTTCATCATG-3′	657	0		218
		Rev 3′-TCCAGATATAGTACATGTAAAGTG-3′		Rev 3′-TCCAGATATAGTACATGTAAAGTG-3′

^aORF, open reading frame.