TABLE 6.

Polymorphic sites within FCA1^a

Strain or isolate no.	5-FC MIC (mg/liter)	Codon position, codon, and amino acidf
		2	11	28	29	34	36	44	70	73	128	141
Reference	0.12	ACG	ATC	GGT	TCA	TCC	GGC	AAC	AAG	AAG	GAA	CCG
		Thr	Ile	Gly	Ser	Ser	Gly	Asn	Lys	Lys	Glu	Pro
1	0.06		GTT			TCT	GAC		AAA	AAA		CCA
2	0.06		RTY			TCT	GRC		AAR	AAA	GWA	CCA
3	0.12
4	0.12		RTY			TCT			AAR	AAA	GWA	CCA
5	0.5
6	0.5		GTT			TCT	GAC		AAA	AAA		CCA
7	1		GTT			TCT	GAC		AAA	AAA		CCA
8	1							AAY
9	1		GTT			TCT	GAC		AAA	AAA		CCA
10	2					TCT	GAC		AAA	AAA		CCA
11	4	ACA			TTA							CCA
12	8		GTT			TCT	GAC		AAA	AAA		CCA
13	16		GTT			TCT	GAC		AAA	AAA		CCA
14	>64		GTT			TCT	GAC		AAA	AAA		CCA
15	>64		GTT			TCT			AAA			CCR
16	>64	ACA		GAT								CCA
17	>64		GTY			TCY	GRC		AAR	AAR
18	>64		RTY			TCY	GRC		AAR	AAR		CCR
19	>64		RTY			TCY	GRC		AAR	AAR		CCR
20	>64		GTT			TCT	GAC		AAA	AAA		CCA
21	>64
22	>64		GTT			TCT	GAC		AAA	AAA		CCA
23	>64		GTT			TCT	GAC		AAA	AAA		CCA
24	>64		GTT			TCT	GAC		AAA	AAA		CCA
		Thrb Thrc	Ileb Vale	Aspd	Leud	Serb Serc	Glyb Aspd	Asnb Asnc	Lysb Lysc	Lysb Lysc	Glub Vald	Prob Proc

^aFCA1 is the gene for cytosine deaminase.

^bSame amino acid and codon as reference strain.

^cSame amino acid as reference strain, different codon.

^dRadical amino acid replacement.

^eConservative replacement.

^fPolymorphic nucleotides are underlined. R = A or G; Y = C or T, W = A or T. The codon and corresponding amino acid associated with 5-FC resistance are shown in boldface type. The codon and corresponding amino acid associated with intermediate 5-FC resistance are shown in italics. Blank spaces indicate the codon is the same as in the reference strain.