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. 2004 Nov;24(22):9863–9872. doi: 10.1128/MCB.24.22.9863-9872.2004

FIG. 1.

FIG. 1.

The expression of ETO in preadipocytes is inhibited during adipogenesis. ETO mRNA expression was determined by Northern blotting (A and B) or real-time PCR analysis (C) of RNA samples extracted from 2-day postconfluent 3T3-L1 (A and C) or 3T3-F442 (B) preadipocytes treated for the times indicated with differentiation mixture. (D) Protein samples extracted from 3T3-L1 preadipocytes incubated with differentiation mixture for various times were Western blotted with and anti-ETO polyclonal antibody (Santa Cruz Biotechnology). (E) RNA isolated from rat whole adipose tissue (WAT), cells of the stromovascular fraction (SVF), or mature isolated adipocytes (MAd) was analyzed by Northern blotting to determine ETO expression. Blots were reprobed with a ribosomal probe to control for loading. A representative blot is shown (upper panel) along with mean data ± the SEM from four rats (lower panel). (F) RNA was isolated from cells of the stromovascular fraction (SVF) or mature isolated adipocytes (MAd) from subcutaneous human adipose tissue samples. MTG8 expression was determined by using real-time PCR and normalized to 18S mRNA. EtBr, ethidium bromide.