FIG. 4.

ETO inhibits the induction of adipogenic gene expression. RNA was isolated from cells retrovirally transfected with GFP (▪), GFP-ETO (□) or GFP-ETO-AA (░⃞) that had been induced to differentiate for 0 to 12 days (D0 to D8) as indicated. (A) Expression of mRNA encoding C/EBPα was determined by Northern blotting. A representative blot is shown along with 18S rRNA, which was used as a loading control and all values were adjusted accordingly. C/EBPα mRNA expression was quantified in four independent experiments, and the mean ± the SEM is shown in the lower panel. Values were expressed as percentage of those in mock-transfected cells at day 12. Asterisks indicate a statistically significant difference from mock transfected cells at the same time point (P < 0.05). (B) Samples from cells differentiated for 3 days were also assayed for mRNA encoding PPARγ₁, PPARγ₂, aP2, and Glut-4 by real-time PCR. The data are means ± the SEM of four independent experiments, and values are expressed relative to that in mock-transfected cells. Asterisks indicate a statistically significant difference from mock-transfected cells (P < 0.05).