FIG. 6.

ETO but not ETO-AA selectively inhibits C/EBPβ transcriptional activity. HepG2 cells were transfected with either a C/EBPα promoter-luciferase reporter construct (A and B) or a minimal C/EBP responsive reporter construct C/EBPwt-LUC (D) with or without C/EBPβ in the absence or presence of increasing quantities of ETO (A, B, and D) or ETO-AA (B) as indicated. (C) Cells were transfected with a PPRE-luciferase reporter construct alone or in combination with PPARγ and increasing quantities of ETO. In each case data shown are means ± the SEM of four independent experiments. Asterisks indicate statistically significant difference from activity in the presence of C/EBPβ (A, B, and D) or PPARγ (C) alone. In panel D, a dagger (†) indicates no significant difference from activity in cells transfected with neither C/EBPβ nor ETO.