FIG. 7.

Effect of 4-OHT treatment on BaF3 and BaF3 · FoxO3a(A3):ER cells. BaF3 and BaF3 · FoxO3a(A3):ER cells were treated with 4-OHT for the indicated times. (A) Cell lysates were prepared at the times indicated, separated on polyacrylamide gels, and subjected to immunoblotting with specific antibodies. The expressions of cyclin D2, BCL6, FoxO3a, and tubulin were analyzed by Western blotting. (B) Total RNA was extracted and analyzed for expression of cyclin D2 and BCL6. Also shown is the expression of GAPDH mRNA as a control for RNA loading and blotting. (C) The expression of BCL6 mRNA was also analyzed in parallel by real-time PCR and normalized to the level of L19 RNA. The results shown are averages of triplicate results from two independent sets of samples.