TABLE 1.
Strains used in this study
| Yeast strain | Genotype | Source |
|---|---|---|
| KP277 | h− ura4-D18 leu1-32 his3-D arg3-D4 | |
| KP106 | h− bub3Δ::ura4+ leu1-32 ade6 ura4-D18 | |
| AE247 | h− mad1Δ::ura4+ leu1 | T. Matsumoto |
| DM075 | h− mad2Δ::ura4+ | T. Matsumoto |
| DM001 | h− mad3Δ::ura4+ | |
| KP175 | h− mph1Δ::ura4+ | S. Sazer |
| KP101 | h+ bub3-Myc::G418 ura4-D18 leul-32 | |
| KP200 | h− bub3-Myc::G418 bub1 HA mad3-GFP::his3+ nda3KM311 | |
| KP258 | h+ bub3-GFP::his3+ ura4-D18 leu1-32 | |
| KP333 | h− bub3-GFP::his3+ bub1Δ::ura4+ | |
| KP330 | h− bub3-GFP::his3+ mad1Δ::ura4+ | |
| KP331 | h− bub3-GFP::his3+ mad2Δ::ura4+ | |
| KP332 | h− bub3-GFP::his3+ mad3Δ::ura4+ | |
| KP334 | h− bub3-GFP::his3+ mph1Δ::ura4+ | |
| KP187 | h+ mad1-GFP::his3+ | |
| KP182 | h− mad1-GFP::his3+ bub1Δ::ura4+ | |
| KP184 | h− mad1-GFP::his3+ bub3Δ::ura4+ | |
| KP180 | h− mad1-GFP::his3+ mad2Δ::ura4+ | |
| KP181 | h− mad1-GFP::his3+ mad3Δ::ura4+ | |
| KP186 | h− mad1-GFP::his3+ mph1Δ::ura4+ | |
| KP252 | h+ mad2-GFP::his3+ | |
| DM059 | h+ mad3-GFP::his3+ | |
| KP156 | h− mad1-GFP::his3+ nda3 | |
| RV001 | h− mad2-GFP::his3+ nda3 | |
| DM076 | h− mad3-GFP::his3+ nda3 | |
| RV002 | h− bub3-GFP::his3+ nda3 | |
| KP379 | h− Ch16 (bub1Δ::ura4) | |
| KP380 | h− mad3Δ::ura4 Ch16 (bub1Δ::ura4) | |
| KP378 | h− bub3Δ::ura4 Ch16 (bub1Δ::ura4) | |
| KP349 | h+lys1 ura4 leu1 ade6-M210 cen2D107(::Kan-ura4-lacO) his7::lacI-GFP | Y. Hiraoka |
| KP383 | h−bub3Δ::ura4 cen2D107(::Kan-ura4-lacO) his7::lacI-GFP | |
| KP384 | h−mad3Δ::ura4 cen2D107(::Kan-ura4-lacO) his7::lacI-GFP | |
| KP382 | h−Mad3-GFP::his3+Cdc11-CFP::G418 | |
| KP381 | h−Bub3-GFP::his3+Cdc11-CFP::G418 | |
| JPJ1660 | h+bub1-K762M | |
| JPJ1821 | h+bub1 Δ28-160 | |
| 1297 | h−ade6-210 his1-102 leu1-32 ura4-DS/E | R. Allshire |
| 4536 | h+sim4-193 TM1::arg3 TM3::ade6 otr2::ura4 his3tel1L ade6-210 leu1-32 ura4D18 arg3-D4 his3-D1 | R. Allshire |
| 2221 | h−TM1(NcoI)::arg3 ade6-210 arg3-D4 his3-D1 leu1-32 ura4-D18 | R. Allshire |
| yVV23 | h+bub1-GFP::his3+his3−arg− | |
| yVV29 | h+ bub1-GFP::his3+ mph1Δ::ura4+ | |
| yVV30 | h−bub1-GFP::his3+bub3Δ::ura4+ | |
| yVV34 | h− bub1-GFP::his3+ mad1Δ::ura4+ | |
| yVV35 | h− bub1-GFP::his3+ mad3Δ::ura4+ | |
| yVV39 | h−bub1-GFP::his3+mad2Δ::ura4+ | |
| yVV40 | h− bub1-K762M bub3-Myc::G418 | |
| yVV41 | h− bub1-GFP::his3+ bub3-Myc::G418 | |
| yVV192 | h− bub1Δ::ura4+ his3− arg3− | |
| yVV194 | h− mad2Δ::ura4+ his3− arg3− | |
| yVV195 | h− mph1Δ::ura4+ his3− arg3− | |
| yVV228 | h− bub1Δ::ura4+ TM1(NcoI)::arg3 | |
| yVV229 | h− mad2Δ::ura4+ TM1(NcoI)::arg3 | |
| yVV230 | h− mph1Δ::ura4+ TM1(NcoI)::arg3 | |
| yVV241 | h− bub1Δ28-160 bub3-Myc::G418 | |
| yVV273 | h− bub1-K762M bub3Δ::ura4+ | |
| yVV276 | h− bub1-Δ28-160-GFP::his3+ arg3− | |
| yVV283 | h− bub1-Δ28-160 mad3-GFP::his3+ bub3-Myc::G418 | |
| yVV206 | h− cdc25 Bub1-GFP::his3+ cut12-CFP::G418 | |
| yVV383 | h− cdc25 Bub1Δ28-160-GFP::his3+ cut12-CFP::G418 | |
| yVV400 | h− bub1Δ::ura4 cen2D107(::Kan-ura4-lacO) his7::lac1-GFP | |
| yVV398 | h− bub1-K762M cen2D107(::Kan-ura4-lacO) his7::lac1-GFP | |
| yVV401 | h−bub1Δ28-160::ura4 cen2D107(::Kan-ura4-lacO) his7::lac1-GFP |