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. 2004 Nov;24(22):9752–9762. doi: 10.1128/MCB.24.22.9752-9762.2004

FIG. 3.

FIG. 3.

TRE17(long) and Arf6 cooperate to induce a constitutively active Arf6 phenotype. HeLa cells were transfected with wild-type Arf6 (A) or Q67L (C) and then subjected to indirect immunofluorescence microscopy with anti-Arf6 antibody. For C, F-actin was visualized with fluorescein isothiocyanate-phalloidin (bottom). (B, D, and E), HA-TRE17(long) and wild-type Arf6 were cotransfected. In B, the two proteins were detected with anti-HA and anti-Arf6 antibodies, respectively. (D) Live cells were labeled with anti-major histocompatibility complex type I for 2 h prior to fixation. (E) F-actin was visualized with fluorescein isothiocyanate-phalloidin. Scale bar, 10 μm in A, C, and E; 5 μm in B and D. (F) Cells were transfected with wild-type Arf6 alone or with TRE17(long) or TRE17(onco). Numbers represent the percentage of cells coexpressing Arf6 and the indicated TRE17 peptide that contain vacuoles. The data represent the results of at least three independent experiments in which 100 cells were counted per experiment.