FIG. 5.

TRE17 vesicles specifically accumulate cargo of the Arf6 pathway. (A) Live HeLa cells expressing HA-T17(447) were incubated with anti-major histocompatibility complex type I for 2 h. Samples were fixed and subjected to indirect immunofluorescence microscopy, visualizing internalized anti-major histocompatibility complex type I (red) and T17(447) (green). (B) HeLa cells expressing T17(447) (green) were incubated with anti-β1-integrin (red) and Alexa Fluor 488-transferrin (blue) for 2 h prior to fixation. Scale bar, 10 μm.