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. 2004 Nov;24(22):9752–9762. doi: 10.1128/MCB.24.22.9752-9762.2004

FIG. 8.

FIG. 8.

TRE17 induces plasma membrane recruitment of Arf6 and cooperates with GEFs to promote activation. Arf6 T27N was expressed by itself (A) or with HA-T17(447) (B and C) in HeLa cells. (B) Representative image of plasma membrane-localized Arf6 T27N upon coexpression with T17(447). (C) Complete redistribution of Arf6 T27N to the plasma membrane was observed in a fraction of cells coexpressing T17(447). Scale bar, 10 μm. (D) Arf6 T27N was expressed by itself or with the indicated TRE17 peptide. The percentage of cells exhibiting plasma membrane (P.M.)-localized Arf6 T27N as typified in B was quantified. Results represent three independent experiments in which 100 cells were counted in each. (E) HA-Arf6 was cotransfected with the indicated plasmids into HeLa cells. GGA3 pulldown assays were performed as above.