FIG. 5.
Mitochondrial function in wild-type and HtrA2/Omi knockout animals. (A) Activities of mitochondrial complexes I, II and III, and IV corrected for mitochondrial enrichment by normalizing the activity against the mitochondrial matrix enzyme citrate synthase; values for detected citrate synthase activities are also shown. Extracts were prepared from striata of P30 control (+/+) and HtrA2/Omi knockout (−/−) animals. A statistically significant (t test analysis) decrease in the amount of citrate synthase activity was detected in samples from HtrA2/Omi−/− animals. (B) Ultrastructural features of mitochondria present in cells from wild-type and HtrA2/Omi knockout animals. Scale bars correspond to 500 nm. (C and D) Quantification of mitochondrial morphological abnormalities detected in cells before and after treatment with mitochondrial stress stimuli. Results are representative of three independent experiments performed on three to five different cell lines isolated from different animals. Rot, rotenone; unt, untreated.