FIG. 8.

Analysis of HtrA2/Omi Smac/DIABLO double-knockout animals. (A) Detection of the wild-type and mutant HtrA2/Omi and Smac/DIABLO loci in the offspring of animals with a heterozygous deletion of the genes for both HtrA2/Omi and Smac/DIABLO. PCR was performed on genomic tail DNA templates. (B) Average body weights of Smac/DIABLO^+/+ HtrA2/Omi^+/+ (wild-type [wt]) and Smac/DIABLO^−/− HtrA2/Omi^−/− (double-knockout [KO]) mice. (C and D) The sensitivities of wild-type and double-knockout MEFs to TNF-α (C), and etoposide (D) are shown. Viability was determined 16 h following treatment by flow cytometry using propidium iodide. Results show the means ± standard deviations of results from three independent experiments.