Skip to main content
. 2004 Nov 1;18(21):2686–2697. doi: 10.1101/gad.1238604

Figure 1.

Figure 1.

σE activity and RseA degradation in ΔrseB strains. (A) Relative σE activity in the wild-type (CAG16037), ΔrseB (CAG22951), ΔdegSsup+ (CAG33315), ΔdegSsup+ ΔrseB (CAG51021), and ΔdegS ΔrseB (CAG51025) strains grown in LB at 30°C. Samples were assayed for σE activity by monitoring β-galactosidase activity produced from a single-copy [ΦλrpoH P3::lacZ] fusion. The differential rate of lacZ synthesis was quantified as described in Materials and Methods. (B) RseA stability in the wild-type (filled squares), ΔrseB (open circles), and ΔdegS ΔrseB (gray triangles) strains. Cells were grown in supplemented M9 media at 30°C to O.D.450 ∼ 0.3, pulse-labeled with [35S]methionine followed by chase of cold methionine. The stability of RseA was determined as described in Materials and Methods.