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. 2004 Nov 1;18(21):2686–2697. doi: 10.1101/gad.1238604

Figure 3.

Figure 3.

The role of the RseP PDZ domain and DegS in inhibiting σE activity. (A) Effects of the presence of RseB and overexpression of a catalytically dead DegS mutant DegS-S201A (pDegS*) on σE activity in cells with RseP ΔPDZ. σE activity in various strains grown in LB at 30°C was determined as described in Figure 2. (White bar) ΔdegSsup+ ΔrseP cells carrying pRseP plasmid (CAG51143); (gray bars) ΔdegSsup+ ΔrseP (CAG51149) and ΔdegSsup+ ΔrsePΔrseB (CAG51147) cells carrying pRsePΔPDZ plasmid and pSU21 vector; (light-gray bars) ΔdegSsup+ ΔrseP (CAG51150) and ΔdegSsup+ ΔrsePΔrseB (CAG51148) cells carrying pRsePΔPDZ and pLC261 plasmids. (B) σE activity increases when RsePΔPDZ is induced. σE activity in the ΔdegSsup+ ΔrseP strain carrying pRsePΔPDZ plasmid and pSU21 vector (CAG51149) grown in LB at 30°C determined as described in Figure 2A, with (+IPTG) or without (-IPTG) overexpression of RsePΔPDZ. (C) Effect of overexpression of DegSS201A on σE activity induced by overexpression of RseP. σE activity in cells grown in supplemented M9 medium in the presence of IPTG at 30°C was determined as described in Figure 2A. (White bar) ΔdegS ΔrseB cells carrying pTrc99a and pSU21 vectors (CAG51120); (gray bar) ΔdegS ΔrseB cells carrying the pRseP plasmid and pSU21 vector (CAG51115); (light-gray bar) ΔdegS ΔrseB cells carrying pRseP and pLC261 encoding DegSS201A (CAG 51116)