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. 2017 Jan 23;7:41138. doi: 10.1038/srep41138

Figure 3. Prostate tumors grown from Cav1-silenced PC3 cells were accompanied by a more reactive tumor stroma.

Figure 3

(A) Tumors derived from shCav1 PC3 cells [PC3(−)] as well as from PC3 shCtrl control cells [PC3(+)] with normal Cav1 expression were removed when tumor volumes reached a critical size (8–14 days after implantation) and were then subjected to immunohistochemistry with the indicated antibodies. Masson´s Goldner Trichrome (TC) was performed in order to visualize the collagenous stroma. Representative images are shown. Sections were counterstained using hematoxylin. Magnification Cav1, TC, FAP, Tagln 20x; Pcna 10x. (B) Subcutaneously grown tumors were further analyzed by immunofluorescence and confocal microscopy. Tumor stroma was stained for smooth muscle actin (ACTA2; red) and Cav1 (green). Arrows point towards Cav1-positive PC3(−) epithelial cells which were supposed to become immunoreactive for Cav1 upon tumor progression. Representative images from at least three independent experiments are shown. Magnification 63x (scale bar 50 μm).