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. 2017 Jan 23;7:41138. doi: 10.1038/srep41138

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Copyright © 2017, The Author(s)

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Paraffin-sections of human prostate tumors were stained for the indicated antibodies. Gleason grading scores used to evaluate prognosis of men with prostate cancer were divided into low (1 + 1, 2 + 2), intermediate (3 + 3, 4 + 3) and high scores (4 + 5) according to the sum of the primary and secondary Gleason patterns in whole resection specimens. The observed patterns of the tumor specimen were assigned based on current WHO and updated ISUP criteria: the primary grade - assigned to the dominant pattern of the tumor (has to be greater than 50% of the total pattern seen) as well as a secondary grade - assigned to the next-most frequent pattern (has to be less than 50%, but at least 5%, of the pattern of the total cancer observed). Asterisks mark stromal compartments and bold arrows point to epithelial structures. Sections were counterstained using hematoxylin. Representative images are shown. Magnification 20x.