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. 2017 Jan 23;7:41173. doi: 10.1038/srep41173

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Copyright © 2017, The Author(s)

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(a) Reduced phosphorylation of endogenous LARP6. HLFs were treated with DMSO (CON), rapamycin (RAPA) and Torin 1 (TOR), and analyzed by Western blot. Arrows: phosphorylated isoforms of LARP6. Loading control: β-actin (ACT). (b) Rapamycin changes pI of LARP6. Cell lysates of HLFs treated with CON or RAPA were subjected to 2DGE followed by Western blot. The scale at the top indicates the pH. (c) Removal of phosphates from LARP6 by calf intestinal phosphatase (CIP). HA-LARP6 was immunoprecipitated and digested without (CON) or with CIP and analyzed by Western blot. (d) Change in pI of LARP6 by CIP. The samples from (c) were analyzed by 2DGE and Western blot.