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. 2017 Jan 3;114(3):E337–E346. doi: 10.1073/pnas.1614926114

Fig. 6.

Fig. 6.

The oral microbiota in Gas6−/− and Gas6+/+ mice is terminally defined during maturation to adulthood and is not altered by cocaging. (A) Adult Gas6−/− and Gas6+/+ mice were cohoused or housed separately. Two months later, oral swabs were taken from the mice to quantify total bacteria by RT-qPCR of the 16S rRNA gene. Bar graphs present the 16S/18S ratio in each group and represent the mean values of five mice ± SEM. To examine bacterial composition, the levels of certain bacterial families were quantified using specific primers for their ribosomal 16S genes. Pie charts represent the average relative frequency of each bacterial family examined. (B) Oral swabs were taken from 2-wk-old and adult (8 wk old) mice and total bacteria as well as the diversity of certain bacterial families were determined and presented as described above. (C) Bar graph depicts the relative mRNA levels of various immunological molecules in the gingiva of Gas6+/+ and Gas6−/− mice using RT-qPCR and represents the mean of five mice ± SEM. Representative data of one of two independent experiments are shown.