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. 2017 Jan 3;114(3):E337–E346. doi: 10.1073/pnas.1614926114

Fig. S6.

Fig. S6.

Age-associated periodontitis in Gas6+/+ and Gas6−/− mice. (A) Gingival tissues were excised from individual mice and total DNA was prepared. Total bacteria were enumerated by RT-qPCR of the 16S rRNA gene. Bar graphs present the 16S/18S ratio in each group and represent the mean values ± SEM (n = 5). (B) Pie charts represent the average relative frequency of each bacterial family examined. (C) Bar graphs present the relative distribution of each bacterial family in the gingiva of Gas6+/+ and Gas6−/− mice. (D) Bar graph depicts the relative mRNA levels of Rankl and OPG in the gingiva of young and aged (20–24 mo old) Gas6+/+ and Gas6−/− mice, representing the mean of five mice ± SEM (n = 5). (E) Representative images of the alveolar bone (both buccal and palatal aspects) of young and aged Gas6+/+ and Gas6−/− mice. Arrows indicate areas in which the tooth root is exposed due to an extensive bone loss. (F) Representative μCT sections of the second upper molar demonstrating the impact of age on bone structure. CEJ, cementoenamel junction; ABC, alveolar bone crest. (G) A 3D quantification of the residual alveolar bone. Data are presented as the volume of alveolar bone in the buccal plate and represent the means of five mice per group ± SEM.